Cell Cryopreservation & Thawing Techniques

We've run extensive tests to determine and understand how the timing of cell isolation and cryopreservation impact immune cell viability and functionality.

When immune cells are carefully and methodically cryopreserved with consistent processes, researchers have greater flexibility in planning their experiments, controlling for certain variables, and making the best and most efficient use of their precious biomaterials. In most cases, the advantages of cryopreserved immune cells far outweigh any downsides.

Tips for Cryopreserving and Thawing Immune Cells

1) Select the Proper Medium

A simple medium that can be prepared with clinical-grade materials is best. Using an albumin concentration that is typically found in serum allows you to mimic the actual serum environment. Prepare the cryopreservation medium in two components: One that is PBS with 2% albumin, and one that is PBS with 2% albumin and 20% DMSO.

2) Cryopreserve Rapidly

We recommend cryopreserving cells as soon as possible following collection. Timing of cryopreservation is critical to ensuring post-thaw viability. Remember that viability will dip slightly after thawing.

3) Aliquot Quickly

Try to aliquot quickly to get your samples in the freezer as fast as possible. Avoid aliquoting in large batches so you can get each smaller batch to the freezer promptly.

4) Freeze at a Controlled Rate

Control your freeze rate at 1°C per minute, per standard recommendations. This will help you avoid freezing the cells too rapidly which can cause ice crystals to form. You can use insulated containers to help control the freeze rate, or purchase a controlled rate freezer.

5) Thaw Rapidly

After you've taken the care to cryopreserve your cells properly, the thawing technique is equally as important to ensure cell viability. Follow our proven cell thawing protocol found here.

This post originally appeared on the Astarte Biologics blog and has been modified for Patch.